Bifidobacterial Endoglycosidases Release Complex N‐linked Glycans from Host Glycoproteins

Chemical and enzymatic release of glycans from glycoproteins.

Ammonium containing buffers should be avoided during enzymatic release of glycans from glycoproteins when followed by reducing terminal derivatization.

the different specificity of the enzymes. In addition, the similarity at the HCA level of the SialylmotifS extends beyond the region defined by linear alignments. We did not find any sequence similarity in the NH2~ te il region, but analysis of the COOH-terminal region showed other conserved regions (see Figure 1). Firstly, considering the same enzyme obtained from various mammalian species, we...

Predominant occurrence of truncated complex type N-glycans among glycoproteins in mature red tomato.

In this study, we analyzed the structures of complex type N-glycans linked to glycoproteins in mature red tomato to determine the relative ratio of high-mannose type structure and complex type structure. Structural analysis of pyridylaminated N-glycans revealed that the truncated plant complex type structure accounted for about 70% of total conjugated N-glycans, while the high-mannose type stru...

Role of complex asparagine-linked glycans in the allergenicity of plant glycoproteins.

Many plant proteins, particularly those found in foods and pollen, are known to act as sensitizing agents in humans upon repeated exposure. Among the cereal flour proteins involved in asthmatic reactions, those members of the alpha-amylase inhibitor family which are glycosylated, polypeptides, BMAI-1, BTAI-CMb*, and WTAI-CM16* are particularly reactive both in vivo and in vitro. We show here th...

A Critical Evaluation of Bifidobacterial Adhesion to the Host Tissue

Bifidobacteria are common inhabitants of the human gastrointestinal tract that, despite a long history of research, have not shown any pathogenic potential whatsoever. By contrast, some bifidobacteria are associated with a number of health-related benefits for the host. The reported beneficial effects of bifidobacteria include competitive exclusion of pathogens, alleviation of symptoms of irrit...